Part:BBa_K1195000:Design
Dispersin B
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 970
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed primers to remove the signal sequence from this nucleotide sequence to allow for intracellular expression of the protein.
Source
We amplified the Dispersin B gene from iGem part BBa_K802001 received from the Lyon-INSA iGem team. Although Dispersin B had several entries in the iGEM registry, no physical part had been submitted until our submission.
References
Pitts, B.; Hamilton, M.; Zelver, N.; Stewart, P. A Microtiter-Plate Screening Method for Biofilm Disinfection and Removal. J. Micro Methods 2003, 54, 269-276.
Kaplan, J. Therapeutic Potential of Biofilm-Dispersing Enzymes. Int. J. Artif. Organs 2009, 32, 545-554.
Ragunath, C.; Shanmugam, M.; Bendaoud, M.; Kaplan, J.; Ramasubbu, N. Effect of a Biofilm-Degrading Enzyme from an Oral Pathogen in Transgenic Tobacco on the Pathogenicity of Pectobacterium Carotovorum Subsp Carotovorum. Plant Pathol. 2012, 61, 346-354.
Gokcen, A.; Vilcinskas, A.; Wiesner, J. Methods to Identify Enzymes that Degrade the Main Extracellular Polysaccharide Component of Staphylococcus epidermidis Biofilms. Virulence 2013, 4, 260-270.